KRAS and colorectal carcinoma: For this purpose, we used isogenic CRC cells with a different KRAS mutation status: HCT-116 cells with a KRAS activating mutation at codon 13, and the isogenic cells HKH2 cells, in which the mutant KRAS allele is disrupted via homologous recombination [23]; DLD-1 parental cells with a KRAS mutation (G13D) and DLD-1 cells, in which the mutant KRAS allele is knocked-out (Horizon Discovery Group, Cambridge, UK); SW48 parental cells with a wild type KRAS gene and the isogenic SW48 cells with a knocked-in KRAS mutant allele G12D (Horizon Discovery Group).