Next, the expression of NF-κB and IRF7 target genes was investigated by RT-PCR and Western blot in MDA-MB-435-Hyg human breast cancer cells, M13SV1-EGFP-Neo human breast epithelial cells and M13MDA435-1 and -3 hybrid cells that were stimulated with LPS and CpG-ODN for 2, 6, 12, 24 and 48 h. The gene discussed is NFKB1; the disease is breast carcinoma.