Ad (iii), to proof the assumption that reversal of the level of the Gli TFs (GLI1 and GLI3) is able to overcome steatosis we treated isolated hepatocytes from steatotic ob/ob mice with overexpression plasmids for GLI1, GLI2, GLI3 and a combination of GLI1 and GLI3. The gene discussed is GLI2; the disease is steatosis.