In order to find out whether these distinct alterations of the GLI factors are characteristic for steatotic livers, we measured the expression signature of the Gli TFs in isolated hepatocytes from melanocortin-4-receptor-deficient mice (MC4R) and Lepob/obmice, which are characterized by massive steatosis as a result of over-nutrition (Sandrock et al., 2009; Itoh et al., 2011; Trak-Smayra et al., 2011). The gene discussed is GLI1; the disease is steatosis.