To measure the biological roles of leptin in NSCLC cells, we first determined the expression of leptin in NSCLC cell lines including 95C, H460, 95D, A549, H1299, SPC-A-1 and in normal human bronchial epithelial cell HBE by qRT-PCR and western blot. The gene discussed is LEP; the disease is non-small cell lung carcinoma.