We first used human brain cancer D54 and D54-EGFRvIII cell lines, in which D54-EGFRvIII was more malignant due to the constitutive activation of mutated epidermal growth factor receptor (EGFR) associated with the overexpression of wild-type EGFR (wt EGFR)18, 19, 20. This evidence concerns the gene EGFR and brain cancer.