To understand how genetic mutations affect the viability of cancer cells in physical confinement, we performed another study with two glioma brain tumor cell lines, D54 and D54-EGFRvIII, in which D54-EGFRvIII was genetically modified D54 in the presence of both mutated and overexpressed wild-type (wt) EGFR. The gene discussed is EGFR; the disease is cancer.