Similarly, here we find that when melanoma cells as well as human osteosarcoma cells are co-treated with 2 mM of 2-DG and 40 μM of FF, GRP78 is attenuated (Figure 3B, 3D), further confirming that 40 μM of FF is converting cells from aerobic to anaerobic metabolism. The gene discussed is HSPA5; the disease is osteosarcoma.