To verify whether SETD7 is more highly expressed in HCC tumor tissues than in ANLTs and that SETD7 correlates with ZBTB20 and CDKN2D in vitro, TMA IHC staining for SETD7, H3K4me2, ZBTB20, and CDKN2D was performed on the 225 pairs of HCC tumor tissues and ANLTs (Fig 4). This evidence concerns the gene SETD7 and neoplasm.