As expected, compared to wild type gp96, mice immunized with mutant gp96 vaccine exhibited dramatically increased tumor growth (Fig 5D) and tumor burden (Fig 5E) by 70 and 75%, respectively (tumor size (mm3) on the 22nd day after B16 injection: wild type gp96 vs. mutant gp96 or B16 Ag alone, 769.6±24.44 vs. 2053.5±14.06 or 2440.26±113.49) (both P<0.05). This evidence concerns the gene RENBP and neoplasm.