ICAM1 and myocardial ischemia: (29) Similarly a 30-minute of myocardial ischemia by ligation of a coronary artery followed by reperfusion caused a significant myocardial necrosis in wild-type mice, while ICAM-1 knockout mice had less necrotic lesion.(30) The study by Kusterer et al. demonstrated that neutrophil adhesion following ischemia-reperfusion was attenuated by the administration of recombinant soluble ICAM-1 protein (31).