To examine the effects of subcellular localization of S100A11 on CRC cell behaviors, either a duplicated nuclear localization sequence (NLS) motif or a duplicated nuclear export signal (NES) sequence was fused in-frame to amino-terminus of HA-S100A11 to generate NLS-HA-S100A11 or NES-HA-S100A11, respectively. Here, S100A11 is linked to colorectal carcinoma.