In summary, roles of TGH in NAFLD progression were studied in three independent mouse models, 16-week HFD-fed obese mice, hypolipidemic and insulin sensitive PEMT deficient mice, and hyperlipidemic and insulin resistant LDLR deficient mice to represent different phases of NAFLD accompanied with different metabolic disorders. This evidence concerns the gene PEMT and metabolic dysfunction-associated steatotic liver disease.