To investigate the combination effects of isoform-selective PI3K inhibitors and SP600125 on glioblastoma cell motility wound was generated in U-87 MG cells which were then treated with PIK-75 (0.1 μM), TGX-221 (20 μM), or CAL-101 (10 μM) combined with SP600125 (20 μM) for 24 h. Here, PIK3CD is linked to glioblastoma.