As with ISX shRNAi, hepatoma cells transfected with E2F1 shRNAi showed decreased expression of DP1 as well as of a proliferation and anti-apoptotic marker (p65), but increased expression of RB1, p53, Apaf-1, Mcl-1, VSP34, ATG12, and LC-3II. Here, APAF1 is linked to hepatocellular carcinoma.