To examine the response of brachyury-specific CD8+ T cells to chordoma cells, we co-cultured HLA-A2+ UM-Chor1 cells for 24 h with brachyury-specific HLA-matched CD8+ T cells or naïve CD8+ T cells isolated from HLA-matched peripheral blood mononuclear cells (PBMCs) from a normal donor as control CD8+ T cells. The gene discussed is CD8A; the disease is chordoma.