MYLK3 and breast cancer: However, CaM did not exert a protective effect against fasudil-induced dilation, indicating that fasudil did not act via MLCK, whereas bosentan combined with fasudil had an additive effect on BC dilation and resulted in a 1.89-fold (p = 0.01) increase in the mean area compared with the 1.48-fold and 1.47-fold increases with bosentan and fasudil alone, respectively.