To understand the relationship between dysregulated GRIM-19 and aberrant STAT3 in GC cells, we first analyzed phospho-STAT3 (Try705) -pSTAT3 (Y705), an active form of STAT3, and its downstream targets including cyclin D1, Bcl-xL, C-myc and Survivin by Western blot. This evidence concerns the gene STAT3 and gastric cancer.