To investigate the expression of JWA and HER2 in GC tissue, immunoblotting was performed on human fresh tumor and adjacent normal tissue samples from patients with gastric adenocarcinoma (n=10), and immunohistochemical (IHC) staining was performed on a tissue microarray (TMA) of local advanced or metastatic GC patients, including GEJ, (n=128) to detect HER2 and JWA. Here, ERBB2 is linked to gastric cancer.