Conversely, semi-quantitative RT-PCR analysis revealed an increased expression of E-Cadherin in N1 silenced cells compared to negative control (Supplementary Figure 2B) in line with Snail reduction suggesting that Notch1 regulates E-Cadherin levels in HCC by transcriptional and post-transcriptional mechanisms. Here, NOTCH1 is linked to hepatocellular carcinoma.