After following the procedure for sensitization and challenge shown in Supplementary Fig. S1a, we compared OVA-induced allergic asthma between AQP3−/− mice and wild-type (WT) mice by assessing airway inflammation using cell counts and lung sections, by evaluating airway responsiveness to methacholine, and by measuring concentrations of IgE and Th2 cytokines. Here, IGHE is linked to allergic asthma.