The majority of subjects (88%) were captured very early after infection, with 12 subjects in Fiebig stage I, 53 in Fiebig stage II/III, 5 in Fiebig stage IV and 4 in Fiebig V. All but two subjects were infected with HIV-1 subtype B, and all subjects exhibited high viral loads typical of acute infection with a median of 923,000 copies/ml (IQR: 270,378–4,100,000) and a median CD4+ T cell count of 413 cells/ml (IQR: 310–552). This evidence concerns the gene CD4 and infection.