TPMS discovered 40 functionally differential proteins (p < 0.05) with altered activation states between the models compared, four of which were mitochondrial proteins: proto-oncogene tyrosine-protein kinase Src (SRC), SHC-transforming protein 1 (SHC1), TNF receptor-associated factor 3 (TRAF3) in the age assessment; and SRC, SHC1 and mitogen-activated protein kinase 10 (MAPK10) in the T2DM comparison (Supplementary Table S10). Here, MAPK10 is linked to type 2 diabetes mellitus.