For TP53, the use of this highly sensitive targeted next-generation sequencing technique provided novel insights for CH2879 and L2975, which were previously reported to be wild type (Supplementary Table 1).18, 19, 20 In CH2879, a subclonal pathogenic mutation was found with a frequency of 17%, which corresponded to the mosaic staining pattern of nuclear p53 observed in the cell pellet and primary tumor (Supplementary Figure 4). Here, TP53 is linked to neoplasm.