Before determining the amount of CPEB4 in our HCC samples, we first used western blot analysis to compare the specificity of the HPA038394 antibody and our polyclonal and monoclonal (Mo) CPEB4 antibodies [31, 33] with selected tumorous (T) and adjacent non-tumorous (N) liver samples from patients with HCC at different stages (S1-S4) as well as brain and liver tissues from CPEB4-WT and -KO mice (Fig 4A). This evidence concerns the gene CPEB4 and hepatocellular carcinoma.