Furthermore, to evaluate their in vitro safety, the normal human liver cell lines (WRL68) were infected with Ad-ΔB or Ad-ΔB/TRAIL+Ad-ΔB/IL-12 at doses of 20 and 50 MOIs in MTT assay (Fig. 3c), and from 1 to 100 MOIs in crystal violet assay (Fig. 3d), and the collective results showed that Ad-ΔB/TRAIL+Ad-ΔB/IL-12 showed potent cancer cell killing efficacy in HCC cells (Hep3B and Huh7), which was markedly higher to that of Ad-ΔB. The gene discussed is TNFSF10; the disease is cancer.