Furthermore, molecular analysis (Fig. 6a-e) showed that tumor tissues of animals treated with Ad-ΔB/TRAIL+Ad-ΔB/IL-12 combined therapy elicited profound elevation in the mRNA and protein levels of TRAIL and IL-12 transgenes (Fig. 6a-d), as well as in the degree of intratumor viral replication as reflected by the expression level of E1A protein (Fig. 6e); suggesting that the strategy of OAd-mediated TRAIL and IL-12 dual gene transfer exhibited simultaneous and efficient expression of the encoded transgenes and didn’t interfere with viral replication in human HCC tumor tissues. This evidence concerns the gene DHTKD1 and hepatocellular carcinoma.