In agreement with these in vitro results, TUNEL assay, that was applied to detect in situ apoptotic cells in the tumor tissues, revealed that the combination of Ad-ΔB/TRAIL plus Ad-ΔB/IL-12 had resulted in a more significant induction of intratumor apoptosis compared to therapy with Ad-ΔB (Fig. 5a). This evidence concerns the gene TNFSF10 and neoplasm.