Next, to clarify the molecular mechanism whereby Ad-ΔB/TRAIL and Ad-ΔB/IL-12 were cooperatively interacted to promote apoptosis, we measured the expression of two common apoptosis-related proteins, caspase-3 and-8 [18], in the harvested liver tumor tissues of all treatment groups and our data revealed that there was a more significant upregulation of caspase-3 and -8 in Ad-ΔB/TRAIL plus Ad-ΔB/IL-12-treated tumor tissues, compared to therapy with Ad-ΔB. This evidence concerns the gene CASP3 and neoplasm.