Interestingly, miR-424 expression was decreased in SiHa cells after CUL2 overexpression (Figure 3F) and increased after CUL2 silence with siRNA (Figure 3G), suggesting there might be a feedback regulatory loop between CUL2 and miR-424, which consequently results in sustained CUL2 overexpression in HPV16 positive cervical cancer cells. This evidence concerns the gene CUL2 and cervical carcinoma.