PBMC from both patients and healthy controls were stimulated with HD IL-2 for 24 hours and culture supernatants were collected and measured for the levels of pro-inflammatory cytokines including IFN-γ, IL-12p70, GM-CSF, IL-10, TNF-α, IL-1β, IL-8 and IL-6. The gene discussed is CSF2; the disease is Huntington disease.