To examine the roles of the binding ability of LDLR to LDL and VLDL in HCV entry, SR/LD-DKO Huh7 cells expressing either wild-type or mutant LDLR by lentiviral vectors were inoculated with HCVcc at an MOI of 1, and intracellular HCV RNA levels were determined by qRT-PCR at 24 h post-infection (Fig 6C). This evidence concerns the gene LDLR and infection.