PTK2 and infection: To verify that the presence of wild-type LLO would not significantly change the process of internalization, we examined the effects of pharmacological FAK and ROCK inhibition and FHOD1 depletion during very low dose infection with wild-type L. monocytogenes (JAT607) and found that internalization of wild-type L. monocytogenes by HUVEC is strongly FAK-, ROCK-, and FHOD1-dependent (Fig 6A, 6B and 6C).