Notably, the most robust invasion-specific hit in our screen came from siRNA pools targeting the focal adhesion protein integrin beta-1 (ITGB1), which increased the fraction of HUVEC infected and the density of foci in our original screen (Fig 2C, 2D and 2I) and also significantly increased infection of HUVEC with an ActA-deficient strain (S2 Table). This evidence concerns the gene ACTA1 and infection.