CREB1 and ductal breast carcinoma in situ: IHC analysis showed that 14-3-3ζ overexpressing DCIS.COM.ζ tumors had increased phospho-ERK and phospho-CREB levels that correlated with higher LDHA expression compared with DCIS.COM.Vec tumors (Figure 5C) but had no significant effect on total ERK, CREB or 14-3-3ζ expression levels (Supplementary Figure S6).