TRIM29 and nasopharyngeal carcinoma: To search biomarkers for early detection of NPC metastasis, in this study iTRAQ tagging followed by 2D LC-MS/MS was performed to identify DEPs between high metastatic 5-8F and non-metastatic 6-10B NPC cells, some DEPs identified by proteomics were selectively validated, the value of three DEPs (RAN, SQSTM and TRIM29) for predicting NPC metastasis was assessed, and the effects of TRIM29 on NPC cell proliferation, migration and invasion were determined.