To further confirm this finding, we measured the expression levels of BRG1 in lymph node metastases and primary tumors from 180 patients with lymph node metastatic CRC by immunohistonchemistry, after which we rated the amount of BRG1 staining by using an immunoreactive score (IRS) (Figure 1A and 1B). This evidence concerns the gene SMARCA4 and colorectal carcinoma.