Regardless of whether the expression of B7-H3 was upregulated in the SW480-B7-H3 cell line or downregulated in Caco-2-shB7-H3 cell line and whether WB or immunofluorescence was used for the analysis, B7-H3 expression induced the cancer cells to lose their epithelial markers and exhibited mesenchymal characteristics. This evidence concerns the gene CD276 and cancer.