NSG mice were used to establish an orthotopic RCC model by injecting skrc59 CAIX+/PD-L1+/luciferase+ RCC cells under the kidney capsule followed by an i.v. injection of 1.0 × 107 CAR T or untransduced T cells (Day 0) and repeated treatment on Day 17 with a lower dose (2.5 × 106) of the same cells. This evidence concerns the gene CA9 and renal cell carcinoma.