PRKAA1 and breast cancer: We firstly detected expression of AMPK in human mammary cell lines (Figure 1A), including human mammary epithelial (HME) cell lines MCF-10A, and human breast cancer cell lines (MDA-MB-231, MDA-MB-435, MDA-MB-468, MCF-7, T47D, BT-474, BT-483, and SKBR3).