It is noteworthy that mutants in pathogenesis-related genes (e.g., SPI-1, SPI-2 and secreted effectors), and genes associated with the Lipopolysaccharide (LPS) of the outer membrane, are negatively selected in spleen and liver of regular BALB/c mice [48] when compared to tumor tissue (compare Figure 1 and Supplementary Figure S1). This evidence concerns the gene SPI1 and neoplasm.