TMPRSS2 and Familial prostate cancer: In patients without prostate cancer (determine via negative biopsy), the detection of TMPRSS2:ERG via urinary cell analysis was lower than our detection rate via EV analysis and ranged from 7–21% (2/30 (7%) [25], 4/30 (13%) [26], 4/24 (17%) [28], 20/96 (21%) [22] and as low as 6% without prior prostate massage, 15/247 (6%) [29].