Furthermore, IHC assay showed that areas of strong ZFP91 signals displayed intense HIF-1α, VEGF and CD31, whereas areas with knockdown of HIF-1α exhibited lower HIF-1α, VEGF and CD31 staining signals in ZFP91-overexpressing colon cancer cells (Supplementary Figure S2). Here, HIF1A is linked to malignant colon neoplasm.