The result of the dual luciferase assay was coincident with our discovery from immunofluorescence of NF-κB subunit p65, but there was no significant difference between non-syndromic tooth agenesis-causing EDA1 mutants and HED-causing EDA1 mutants (Fig 4Z). The gene discussed is NFKB1; the disease is hypohidrotic ectodermal dysplasia.