In our study, although the HED-causing EDA1 mutant proteins (H252L and Y343C) were unable to bind to their receptors and activate NF-κB in LS8 cells, the BMP4 expression was increased and WNT10A and WNT10B expression were reduced dramatically. The gene discussed is NFKB1; the disease is hypohidrotic ectodermal dysplasia.