The impaired AKT activation and CyclinB1 expression were also observed in FaDu and SCC25 cells transfected with siFRA1 oligonucleotides (Figure 4D), implicating that FRA1-regulation of AKT and CyclinB1 is likely a general mechanism in HNSCC cells. This evidence concerns the gene FOSL1 and head and neck squamous cell carcinoma.