We therefore decided to apply only two rounds of CTL co-culture for the selection of resistant melanoma clones, because under these conditions the HLA-A*0201 and Melan-A/MART-1 expressions of the surviving clones was similar or even higher than those of the parental UKRV-Mel-15a cell line (Fig. 1A). The gene discussed is MLANA; the disease is melanoma.