To test this hypothesis, the five MM cell lines used in Fig. 1 were treated with either 10 μM lenalidomide or AGO2-shRNA-74 and analyzed with an Alexa Fluor 647 Annexin V kit to determine the percentage of live cells, apoptotic cells and necrotic cells (Fig. 8a). This evidence concerns the gene AGO2 and Miyoshi myopathy.