To determine whether the effect of IP6K1 on chemotaxis and migration extends to cancer cells, we generated HCT116 and HeLa cell lines expressing shRNA directed against human IP6K1. Of the five different shRNA constructs tested, we observed > 30% knockdown in IP6K1 expression with only two constructs, which we called shIP6K1–1 and shIP6K1–4. The gene discussed is IP6K1; the disease is cancer.