CREG1 and type 2 diabetes mellitus: Secondly, under high concentrations of glucose (25 mM D-glucose) and different concentrations of palmitate (0.2, 0.3 or 0.4 mM), which simulate the pathological stimuli of poorly controlled T2DM in vivo, apoptosis increased and CREG expression decreased in HUVECs in a concentration dependent manner in vitro.