CDH1 and neoplasm: Moreover, in the study conducted on various tumor type by Bronsert and colleagues, the budding tumor cells displayed loss of cell polarity (i.e., a morphological shift towards rounded and spindle-like phenotype), decreased levels of E-cadherin, decreased membrane localization coupled to increased cytoplasmic localization of E-cadherin, and increased nuclear ZEB1 immunostaining at a single-cell level [10].