In agreement with the above reports, we have seen an enhanced expression of GADD45 in our HCC cells treated with a combination of 5-AZA and vitamin C. It has been reported recently that GADD45 could also induce active demethylation processes via nucleotide excision repair or base excision repair pathway [44] which might also be associated with the induction of active demethylation via 5-AZA and vitamin C, as shown in this study. This evidence concerns the gene GADD45A and hepatocellular carcinoma.