The association of lactate and glutamine catabolism in the regulation of mTOR in these conditions of stressful vascular insufficiency is further substantiated by our results showing that mTOR upregulation can be suppressed by impairing lactate uptake with known inhibitors of MCT1, or by inhibiting enzymes involved in glutaminolysis, namely glutaminase 2 that converts glutamine to glutamate in the first step of its metabolism and an alanine aminotransferase that in turn catalyzes the production of alanine + α-ketoglutarate from (lactate-derived) pyruvate and glutamate. Here, GLS2 is linked to vascular insufficiency disorder.