We performed immunoblotting analysis of PTEN and found much higher levels of PTEN in CE-low HPDE6, AsPC-1 and BxPC-3 cells, but lower levels of PTEN in CE-rich MIA PaCa-2 and PANC-1 cells, suggesting a negative correlation between PTEN and CE level in pancreatic cancer cells (Figure 3a). This evidence concerns the gene PTEN and pancreatic neoplasm.