LAMB2 and muscular dystrophy-dystroglycanopathy, type A: Immunofluorescence and western blot analysis of skeletal muscle from the 18 week fetus (P2) showed an α-DG glycosylation defect similar to previously described glycosylation-deficient WWS patients (Willer et al., 2012) with complete loss of both functional glycosylation and laminin binding (Figure 7—figure supplement 2B,C).