To confirm this result in broadly studied cell lines, we identified two human HCC cell lines (HepG2 and Bel7404) and two mouse hepatoma cell lines (Hepa1-6 and Hepa1c1c7) as representative pERK high expression (pERK+) and low expression (pERK−) cells, respectively, by Western blot of total cell lysates (Figure 1A). The gene discussed is EIF2AK3; the disease is hepatocellular carcinoma.