Patients that were compound heterozygous for RB1 (confirmed by conventional DNA diagnostics) had a mean VAF close to 0.5 (mean 0.48) and patients with homozygous RB1 mutations (confirmed by conventional DNA diagnostics) had a mean VAF close to 1 (mean 0.98), indicating high tumor cellularity in our cohort. This evidence concerns the gene RB1 and neoplasm.